Скачать или смотреть Optimizing Cytokine Analysis Choosing the Right Assay for Accurate Detection and Interpretation of..

Presented By: Laura Hocum Stone and Scott Hunter Oppler


Speaker Biography: Dr. Laura Hocum Stone is a non-clinical applications specialist who leads the in vitro service line for the University of Minnesota’s Preclinical Research Center (PCRC). She received her Ph.D. in neuroscience from the University of Minnesota in 2014 and has deep expertise in animal disease models, cell culture, and immune monitoring reflected in her research publications. PCRC is known for its modeling of complex therapies in primate models of disease in conjunction with a distinctive behavioral management program. To support the Center’s commitment to reduction in animal use and refinement of models where animals are required, our in vitro research focuses on assay characterization, development, and optimization as it relates to enhancing translatability of preclinical research to successful clinical application to improve scientific rigor and reproducibility.

Hunter Oppler is a dual PhD/DVM student at the University of Minnesota College of Veterinary Medicine, enrolled in the college’s Comparative and Molecular Biosciences program, advised by Dr. Melanie Graham. He received his B.S. in Biology and Psychology from the University of North Carolina, Chapel Hill in 2014. The focus of his PhD research has centered around improving the scientific rigor of NHP research towards improved clinical translation and animal welfare. His work focuses on investigating how specific model-related parameters, including handling technique, disease characterization methodology, and disease management, directly affect model validity. A major aspect of this work, compares the effects of restraint versus cooperation for intensive handling necessary in safety and efficacy modeling. This work has been pivotal in the design of experiments to enhance translational relevance and improve animal welfare.


Webinar: Optimizing Cytokine Analysis-Choosing the Right Assay for Accurate Detection and Interpretation of Cytokine Expression


Webinar Abstract: The increasing availability of commercial multiplexed cytokine assays has made measurement of circulating cytokine levels more common in the study of the pathogenesis of disease, but it also introduces several technical challenges that can make assay design more complicated. Determination of the suitability of an assay for a given application as well as a thorough understanding of how elements of experimental design may impact study outcomes are critical to the generation of robust and rigorous research data.

Nonhuman primates (NHPs) are often chosen as a preclinical model of disease and treatment due to their close evolutionary relationship with humans, including a complex immune system. Since these studies are often the last step before translation to clinical application, it’s critical that researchers understand how demographic characteristics and experimental design, such as species and sex, may affect therapeutic efficacy or safety. Confidence in immunoassay data depends on the accuracy and precision that is only possible from using high quality antibody reagents. This means selecting antibodies with not only high analyte affinity but also specificity, which is the ability to distinguish between the analyte and other structurally similar components.

Presenters will highlight their experience using Luminex nonhuman primate cytokine performance panels and the advantages of measuring a multitude of different targets without increasing your sample volume.


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