Скачать или смотреть Methods for Culturing Primary Adult CNS Neurons

Presented By: Erna van Niekerk, Ph.D.

Speaker Biography: Erna van Niekerk earned her Ph.D. from the University of Delaware under the guidance of Dr. Jeffery L. Twiss where she worked on axonal transport and helped identify a novel mechanism that regulates microtubule transport directionality. This novel and mechanistically important work was published in PNAS.

Webinar: Methods for Culturing Primary Adult CNS Neurons

Webinar Abstract: In 1910, Harrison published the first report of frog embryonic sympathetic ganglia grown in hanging drops of lymph for a few days, where single neurons extended nerve fibers with complex growth cones extending and contracting over time towards target cells. The revolutionary ability to culture embryonic and post-natal neurons for extended time periods demonstrated that developing neurons could form plexi of interconnected nerve fibers. However, over the years it has proven to be exceedingly difficult to consistently culture adult central nervous system (CNS) neurons; while several instances of success in culturing fewer than 10 neurons have been reported, a major unresolved methodological challenge in neuroscience has been the ability to culture adult CNS neurons from any region consistently and in tractable numbers.

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