Multiplexed Fluorescent IHC Staining and Analysis in Lymphoma | Protocol Preview

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Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma - a 2 minute Preview of the Experimental Protocol

Guo Hong, Shuangyi Fan, The Phyu, Priyanka Maheshwari, Michal Marek Hoppe, Hoang Mai Phuong, Sanjay de Mel, Michelle Poon, Siok-Bian Ng, Anand D. Jeyasekharan
AnSteel Group General Hospital, Department of Laboratory Medicine; National University of Singapore, Cancer Science Institute of Singapore; National University of Singapore, Department of Pathology, Yong Loo Lin School of Medicine; National University Health System, Department of Haematology-Oncology;

Here we describe a protocol for multiplex fluorescent immunohistochemical staining and imaging for the simultaneous localization of multiple cancer-associated antigens in lymphoma. This protocol can be extended to the colocalization analysis of biomarkers within all tissue sections.

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