Скачать или смотреть Optimize a crystallization reagent using Make Tray

Instructional video showing how to optimize a crystallization reagent using the Make Tray feature on the Hampton Research website.
https://hamptonresearch.com/make-tray...

The example in the video is using GRAS Screen 1, Reagent A1. This crystallization reagent is made up of 0.2 M Ammonium acetate and 30% v/v Polyethylene glycol 300.

A sample optimization screen in a 24 well plate format has been created from the crystallization reagent. This sample optimization screen uses stocks of 8 M Ammonium acetate and 100% Polyethylene glycol 300 to form a grid screen around the crystallization reagent concentrations. The example uses a reservoir volume in microliters of 1,000.

The sample optimization is simply a starting point for optimizing the initial crystallization reagent. You can change any of the fields to fit your needs within a 24 well plate parameter.

Polyethylene glycol 300 can be changed to Polyethylene glycol 400 by typing in the field for "Reagent 2 Name:".

The "Set of concentrations in the X direction: list values separated by spaces" field can be changed from "10 15 20 25 30 35" to "5 10 15 20 25 30" by just deleting the existing numbers and typing in the new ones.

Visit the Make Tray Instructions webpage for more details.
https://hamptonresearch.com/make-tray...