Inducible Expression System: Bacterial VF and Intracellular Signaling | Protocol Preview

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Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling - a 2 minute Preview of the Experimental Protocol

Christian Berens, Stephanie Bisle, Leonie Klingenbeck, Anja Lührmann
Friedrich-Alexander-Universität, Department Biologie; Friedrich-Loeffler-Institut, Institut für Molekulare Pathogenese; Universitätsklinikum Erlangen, Mikrobiologisches Institut - Klinische Mikrobiologie, Immunologie und Hygiene;

The method described here is used to induce the apoptotic signaling cascade at defined steps in order to dissect the activity of an anti-apoptotic bacterial effector protein. This method can also be used for inducible expression of pro-apoptotic or toxic proteins, or for dissecting interference with other signaling pathways.

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