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Genomic abnormalities, mycoplasma detection and cell line authentication are a real challenge for scientists working on human stem cells. Various stressing factors can trigger genetic defects during the cell culture process. Mislabeling or cross-contamination can lead to the wrong identification of the cell lines, and of course, mycoplasma contamination can wreak havoc in your lab. By overlooking these challenges, you can compromise the safety and quality of your research work, its reproducibility and ultimately, the reputation of the field.

The ISSCR raised a red flag and put together some guidelines to try and minimize these issues in the “Standards for Human Stem Cell Use in Research” document produced back in June 2023.

To increase your confidence in the quality of your cell line, Stem Genomics recommends regular genetic stability testing, mycoplasma detection and short tandem repeats throughout the lifecycle of the cells in culture. For other key stages, such as bank characterization, new cell line acquisition and end of process, we recommend adding G-Banding karyotyping to your QC. Finally, after clone screening and during differentiation, it is good practice to monitor pluripotency.

For most labs, this best practice can only be implemented if these tests are performed quickly and within a (reasonable) budget. This is what led Stem Genomics to offer a fast, one-stop-shop service combining several essential quality control assays.