🛑 CALCULATE LIMIT OF DETECTION (LoD) AND LIMIT OF QUANTITATION (LoQ) | Adwoa Biotech

The limit of detection is referring to the lowest amount of your analyte that you can detect, but not necessarily be able to quantify. The limit of quantitation is the lowest amount that you can both detect and quantify with a predetermined precision. There are several types of limit of detection; you may be interested in the instrument detection limit (IDL), the method detection limit (MDL), the practical quantification limit (PQL) or the limit of quantification (LOQ).
Two methods for deterring the limit of detection and quantification limit are presented in this video. The first limit of detection and quantification method presented focuses on measuring blanks, then using mean and standard deviations calculate the limit of detection and limit of quantitation. The second method uses a standard curve (calibration curve) and the regression line to calculate the detection and quantification limits.

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This video is about limit of detection, limit of quantitation, how to calculate LOD, how to calculate LOQ, detection limits, how to calculate LOD and LOQ, quantification limit, method validation, method development, quantitation limit, QC validation of the analytical method, signal-to-noise and signal-to-noise ratio.