BALF Exosomes in Lipopolysaccharide-induced Septic Lung Injury |Protocol Preview

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Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury - a 2 minute Preview of the Experimental Protocol

Zhihong Yuan, Brahmchetna Bedi, Ruxana T. Sadikot
Atlanta VAMC, Department of Veterans Affairs; Emory University, Division of Pulmonary and Critical Care Medicine, Department of Medicine;

Mice exposed to intraperitoneal LPS secrete exosomes in Broncho-alveolar lavage (BAL) fluid that are packaged with miRNAs. Using a co-culture system, we show that exosomes released in the BAL fluid disrupt expression of tight junction proteins in bronchial epithelial cells and increase expression of pro-inflammatory cytokines that accentuate lung injury.

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