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Rapid Genotyping of Animals Followed by Establishing Primary Cultures of Brain Neurons - a 2 minute Preview of the Experimental Protocol

Jin-Young Koh, Sadahiro Iwabuchi, Zhengmin Huang, N. Charles Harata
University of Iowa Carver College of Medicine, Department of Molecular Physiology & Biophysics; University of Iowa Carver College of Medicine, Department of Psychiatry; EZ BioResearch LLC,;

We describe procedures for labeling and genotyping newborn mice and generating primary neuronal cultures from them. The genotyping is rapid, efficient and reliable, and allows for automated nucleic-acid extraction. This is especially useful for neonatally lethal mice and their cultures that require prior completion of genotyping.

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