Скачать или смотреть What is sequencing depth? | Bioinformatics 101

In this video I explain a term frequently used with sequencing i.e. depth associated with coverage (or depth of coverage, sequencing depth or read depth). In addition to discussing what these terms mean, I talk about what is the recommended coverage for different NGS data, how to visualize coverage and how to calculate coverage and coverage metrics from BAM files using samtools.
I hope you find this video helpful! Leave your thoughts in the comment section below!

Chapters:
0:00 Intro
0:32 What is coverage?
1:23 Depth of coverage (or sequencing depth)
1:49 Breadth of coverage
2:32 How to calculate coverage? (Lander/Waterman equation)
4:15 Recommended sequence coverage for NGS data
5:42 Visualizing coverage
6:17 Calculate coverage and coverage metrics

You can show your support and encouragement by buying me a coffee:
https://www.buymeacoffee.com/bioinfor...

To get in touch:
Website: https://bioinformagician.org/
Github: https://github.com/kpatel427
Email: [email protected]

#bioinformagician #bioinformatics #sequencing #coverage #samtools #depthofsequencing #samflag #sam #bam #alignment #phred #fasta #fastq #singlecell #10X #ensembl #biomart #annotationdbi #annotables #affymetrix #microarray #affy #ncbi #genomics #beginners #tutorial #howto #omics #research #biology #GEO #rnaseq #ngs