Quick tips and tricks for resuspending pellets

Start by breaking up big clumps (especially important for gunky things like cell pellets)
Play the eppendorf rack xylophone! Scrape the bottom of the tube across a microcentrifuge tube rack
If you have a lot of pellets such as bacterial cell pellets for mini preps, put them in a rack, put your hand firmly on top so they don’t fly out, then move the rack back and forth over a vortex

Then (or if you’ve skipped the big-break-up, which isn’t needed for easier-to-resuspend pellets), make things homogenous (ensure an even mixture).
Pipet up and down over/above the pellet - create a vortex-like effect in the liquid, giving the pellet a sort of jet-stream bath.
DO NOT TOUCH THE PELLET WITH THE PIPET TIP! If you do, you can get gunk stuck in and/or on the tip. Not only can you lose yield this way, but if it clogs up the tip you can end up making a sort of suction that then breaks and sucks up a bunch of air.
When doing that pipetting up and down (triturating) - set your pipet to a lower volume than there is in the tube. This will help prevent bubble formation.

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