In Situ Hybridization for comparing Gene expression | Protocol Preview

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Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos - a 2 minute Preview of the Experimental Protocol

Giselbert Hauptmann, Iris Söll, Robert Krautz, Ulrich Theopold
Stockholm University, Department of Molecular Biosciences, The Wenner-Gren Institute (MBW);

We describe a multi-target chromogenic whole-mount in situ hybridization (MC-WISH) procedure in intact Drosophila embryos allowing simultaneous and specific detection of three different mRNA distribution patterns by contrasting color precipitates.

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