Preparation of Liposomes from Soy Lecithin [ Liposomal Drug Delivery - Cheap Method ]

Synthesis & Characterization Soybean Phosphatidylcholine Liposome
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Preparation of Liposomes in Nano
Best Cheap method of preparation



Liposomes are spherical vesicles with one or more phospholipid bilayers that surround an aqueous compartment. Liposomes can encapsulate aqueous hydrophilic or hydrophobic substances within the phospholipid bilayer and are often classified as small unilamellar vesicles (SUVs), large unilamellar vesicles (LUVs), or multilamellar vesicles (MLVs), based on their size and the number of phospholipid bilayers. SUVs and LUVs have diameters in the range of 20–100 and 100–1000 nm, respectively, and are often used as biomaterial carriers.

Liposomes are also tools for the oral delivery of nutraceuticals because of their unique properties. Ordinary tablets and capsules have low absorption and bioavailability properties; thus, the encapsulating fat-soluble and water-soluble nutrients in liposomes are investigated for the ability to prevent their destruction in the digestive system and facilitate their delivery to cells and tissues.
Liposomes were aseptically prepared by the thin-film hydration method. The lipid phase components (soy lecithin, cholesterol, CTAB, LET, and PTX) were accurately weighed and dissolved in chloroform : methanol mixture (2 : 1, V/V ), in the ratio of 9 : 1 soy lecithin : cholesterol, 1% CTAB, 5% LET, and 5% PTX.
The solution was transferred to a round-bottom flask and connected to a BUCHI rotavapor R-114 and BUCHI water bath B-480 with an applied vacuum and temperature maintained at 45°C until the complete evaporation of solvents. The obtained dry, thin lipid film was hydrated with 0.5% Tween 80 by stirring at room temperature.
The suspension was sonicated by the probe sonicator for 30 min and was further homogenized by a mini extruder (EmulsiFlex-05 homogenizer, Avestin Inc., Ottawa, Canada) for 10 cycles. The obtained liposomal suspension was centrifuged at 16000 rpm for 30 min to separate the unencapsulated drugs. The resulting formulation was lyophilized and stored at 2-8°C for further analysis.
The size distribution and zeta potential of the liposomal formulations were characterized by dynamic light scattering (DLS) using a Zetasizer Nano SZ (SZ-100, Horiba). The measurement was determined through a helium-neon (He-Ne) laser beam with the detection angle and the temperatures as 90° and 25°C, respectively. Samples were diluted with deionized water prior to measurement to reach the phospholipid concentration of 1000 ppm. The morphology of LET-PTX-Lips was examined by a transmission electron microscope (TEM) using JEM-1400, JEOL (Tokyo, Japan). The sample was diluted with deionized water (1 mg/mL). One drop of the liposomal formulation was deposited onto a carbon-copper grid (300-mesh, Ted Pella, Inc., USA) and air-dried at room temperature.