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HSST/SET Botany Module Syllabus Based Classes - Genetic Markers: RFLP and AFLP Explained

Genetic Markers| RFLP|AFLP| Molecular Markers|DNA Fingerprinting|Botany| HSST|SET 2926|Molecular Biology


Explore the world of genetic markers and learn about RFLP (Restriction Fragment Length Polymorphism) and AFLP (Amplified Fragment Length Polymorphism) techniques. Understand how these molecular markers are used in plant genetics, diversity studies, and breeding programs.

RFLP:
RFLP is a technique that analyzes variations in DNA sequences between individuals or species. It involves digesting DNA with restriction enzymes and separating the resulting fragments based on size.

AFLP:
AFLP is a PCR-based technique that combines the power of RFLP with the sensitivity of PCR. It involves digesting DNA with restriction enzymes, ligating adapters, and amplifying the fragments using PCR.

Applications:
Genetic markers like RFLP and AFLP are used in:

DNA fingerprinting
Genetic diversity studies
Plant breeding programs
Marker-assisted selection

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