Скачать или смотреть nanoHUB-U Biodesign L5.4: Cellular Devices - Cells as Biosensors II

Table of Contents:
00:00 L5.4: Cells as Biosensors II
00:12 In this lecture
00:24 What is a biosensor?
01:17 Sensitivity
01:56 Sensitivity
02:42 Dynamic Range
04:05 Classic Example of Magnitude Amplification - Phototransduction
05:20 Whole Cell Biosensor
07:25 Whole Cell Biosensor from Simple Activation
08:53 Classic Hyperbolic Response (.e. Calibration) Curve
10:55 Sensitivity Amplification
11:53 Ultrasensitivity As greater than 1 for a range of S
15:20 Biological Design Approaches to Achieve Ultra-sensitivity
16:10 Ultra-sensitivity – Cooperativity
17:52 Ultra-sensitivity: Multi-stage Cascades
20:40 Ultra-sensitivity: Zero Order Sensitivity
22:32 Structure is used widely by biology
23:53 0o Ultrasensitivity Depends on Certain Conditions:
25:16 Cascades to Enhance 0o Ultra-sensitivity:
28:30 Engineering Design: Analogy to the Transistor
29:32 Coming up …

This video is part of the nanoHUB-U course "Biological Engineering: Cellular Design Principles" currently offered on nanoHUB-U (https://nanohub.org/courses/biod) or on EdX (https://www.edx.org/course/biological...)

Cellular Design Principles is a five week course that explores the design principles underlying mechanisms of cellular and biomolecular functions such as cell architecture, energy storage and conversion, sensing and signaling, communication, time keeping, molecular synthesis, memory, and motility. Emphasis will be placed on the chemical, physical, and mathematical features that determine the performance of the biological device. Topics cover both cellular/biochemical processes and molecular/genetic circuits. Examples are presented from reverse engineering of natural systems and design of new synthetic systems.