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Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library - a 2 minute Preview of the Experimental Protocol

Luis Gomez-Castillo, Kurumi Watanabe, Huayi Jiang, Shoukai Kang, Liangcai Gu
University of Washington, Department of Biochemistry and Institute for Protein Design;

Creating chemically induced protein dimerization systems with desired affinity and specificity for any given small molecule ligand would have many biological sensing and actuation applications. Here, we describe an efficient, generalizable method for de novo engineering of chemically induced dimerization systems via the stepwise selection of a phage-displayed combinatorial single-domain antibody library.

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