Скачать или смотреть Rory Power: Advanced custom microscopy development for service provision at the EMBL Imaging Centre

Speaker: Rory Power (EMBL)

Summary: Advanced custom microscopes offer unique capabilities unmet by commercial offerings, but often contribute to progress in the biomedical sciences only narrowly, given that they are typically confined to developer labs for whom providing widespread advanced microscopy access is not a core remit. Making these developments more widely available can be achieved by providing access to instrumentation and expertise as a service in imaging facilities and centres. Doing so comes with a number of challenges that are not apparent in specialised developer labs. I will outline the service model at the EMBL Imaging Centre and describe, from an engineering perspective, experiences in setting up two advance microscope. Firstly, an automated, multi-colour, 3D single molecule localisation microscope (3D-SMLM, Power et al., Nature Protocols, 2024) and secondly, a high-numerical aperture oblique plane microscope (OPM), based on the AMS-AGY objective (a.k.a. ’Snouty’, Sapoznik et al. eLife, 2020)