Isolation Of Lipase Producing Bacteria | Tributyrin Agar | Lipid Hydrolysis Assay |

Описание к видео Isolation Of Lipase Producing Bacteria | Tributyrin Agar | Lipid Hydrolysis Assay |

Enzymes that break simple fat into its component fatty acid and glycerol are known as lipases.

Many bacteria can be classified based on their ability to produce and secrete lipases.


tributyrin is the simplest naturally occurring triglyceride and can be used for this determination

Tributyrin is too large to enter the cell, so a lipase is released to break it down prior to cellular uptake.

After hydrolysis, the glycerol can be converted to dihydroxyacetone phosphate, an intermediate of glycolysis...

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