Slide-tags Enables Single-Nucleus Barcoding for Multimodal Spatial Genomics

Combining the high-quality and high-throughput quantification of single-nucleus genomics measurements with the spatial context of tissue measurements comes Slide-tags , an innovative method in which single nuclei in intact tissue sections are tagged with spatial barcode oligonucleotides derived from DNA-barcode beads of known position. Hear from Andrew Russell, Jackson Weir, Naeem Nadaf, and Fei Chen of Broad Institute of MIT and Harvard as they describe the development and application of this impressive technology that only adds 10 minutes to existing single-nucleus RNA sequencing workflows.

Read the article published in Nature:
https://www.nature.com/articles/s4158...

Code availability:
Code for processing spatial sequencing libraries is available at GitHub
https://github.com/broadchenf/Slide-tags (https://doi.org/10.5281/zenodo.2571615)

Fei Chen Lab: https://www.insitubiology.org/
Evan Macosko Lab: https://macoskolab.com/

Learn more about the TDCC and other NHGRI funded projects
https://genometdcc.org/