IsoView: Long-term Time Lapse Recording of Drosophila Embryo Development

Within less than a second, the new IsoView microscope produces images of entire organisms, such as a zebrafish or fruit fly embryo, with enough resolution in all three dimensions that each cell appears as a distinct structure.

This video shows dorsoventral (top) and lateral (bottom) maximum-intensity projections of an IsoView long-term time-lapse recording of a stage 17 Drosophila embryo expressing the calcium indicator GCaMP6s throughout the nervous system. The embryo develops into a first instar larva that crawls out of the imaging volume at the end of the recording. Imaging was performed at 2 Hz over a period of 9 hours. Over 120,000 single-view image volumes (4.5 million images, 9 terabytes) were acquired for this specimen, resulting in more than 30,000 multi-view deconvolved high-resolution IsoView image stacks. The color code shows low and high fluorescence intensity levels in blue and green, respectively. (Video 4)

Video: Keller Lab, HHMI/Janelia Research Campus

News story: https://www.hhmi.org/news/seeing-big-...

Visit the Keller Lab at Janelia: https://www.janelia.org/lab/keller-lab