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Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of Caenorhabditis elegans' Locomotion - a 2 minute Preview of the Experimental Protocol

Justin Tong, Pouya Rezai, Sangeena Salam, P. Ravi Selvaganapathy, Bhagwati P. Gupta
McMaster University , Department of Biology; McMaster University , Department of Mechanical Engineering;

A semi-automated micro-electro-fluidic method to induce on-demand locomotion in Caenorhabditis elegans is described. This method is based on the neurophysiologic phenomenon of worms responding to mild electric fields (“electrotaxis”) inside microfluidic channels. Microfluidic electrotaxis serves as a rapid, sensitive, low-cost, and scalable technique to screen for factors affecting neuronal health.

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