Biacore Surface Plasmon Resonance: Observing Molecular Interactions in Real-Time

The Biophysics Instrumentation Core (BIC) Facility offers sophisticated instrumentation dedicated to characterizing biomolecular conformations and interactions. Biacore surface plasmon resonance (SPR) instruments measure molecular interactions in real time, allowing determination of kinetic and affinity parameters for specific interactions. Biacore instruments are routinely used to measure the interaction of target molecules to binding partners as small as 100 Da or as large as intact cells. Through examination of general principles of SPR and specific case examples, seminar attendees will learn how Biacore instruments such as the Biacore T200 at the Biophysics Instrumentation Core facility of the University of Notre Dame may prove useful for their ongoing research.