Rooting Success Rate of cannabis Cuttings Research

Rooting Success Rate of Cannabis Cuttings Research
Professor DeBacco


Research ArticleCaplan, Deron & Zheng, Youbin & Dixon, Mike. (2018). Propagation and Root Zone Management for Controlled Environment Cannabis Production.https://www.researchgate.net/publicat... Variables


Growers often wonder what the impact would be of different rooting conditions.If conditions are favorable there will very likely be some degree of rooting.However the goal for growers is to maximize production.


Cannabis Plant Material and Factors Explained
Cannabis [Cannabis sativa L. ‘WP:Med (Wappa)’] cuttings were taken at a length of ≈13 cm and with three fully-expanded leaves from stock plants. Cuttings were taken from the ends of axial limbs and cut at a 45˚ angle.Each cutting was rooted in a 5.7 cm wide, 5.7 cm tall peat-based “Jiffy” pot containing Pro-Mix PG Organic growing substrate Full factorial completely randomized design with four factors (rooting hormone, leaf number, cutting position, and leaf tip removal), two levels per factor and 10 replications per factor combination. For leaf number: cuttings had either one fully-expanded leaf removed (two leaves remaining) or were left with three leaves. For cutting position: cuttings from terminal shoots were taken from either an apical position (node 10 and higher) or a basal position (below node 10). For the leaf tip removal treatment: a portion of leaf tips (≈1/3 of the leaf area) was removed from the fully expanded leaves or the leaves were left uncut. For the rooting hormone factor: the base (≈5 cm) of each stem was dipped in either 0.2% indole-3-butyric acid gel or in a 0.2% willow extract rooting gel


Propagation Environment
Trays were randomly arranged in a walk-in growth chamber and initially misted.Relative HumidityDays 0-4 = 95%Days 5-8 = 80%Days 9-12 = 60%Temperature was maintained at 24 ˚C (s.d. ±0.04˚C) (day/night) for the entire period. Fluorescent lighting was used to maintain an 18-hour photoperiod.Light Intensity: PAR (µmol m-2 s-1)Days 0-5 = 50Days 6-9 = 80Days 10-12 = 115 Harvest Determination The bottom of the trays was observed daily from 7 DAP on wards for protruding roots, and cuttings were harvested at 12 DAP when approximately more than 50% of the cuttings showed visible roots at the bottom of the tray.




Rooting Success Rate of Cannabis Cuttings (n = 40). Cut leaves had about 1/3 of leaf tips were removed. Cuttings were from terminal shootsApical cuttings taken from greater than or equal to node 10Basal cutting from less than node 10. Bars within each factor bearing different letters are significantly different at P less than 0.05 using chi squared contrasts.


Research Summary Rooting hormone: 0.2% IBA gel delivered higher rooting success rate than the 0.2% willow extract. Cutting 30% of Leaf Tips: Reduced rooting success rate Number of Leaves: Three leaves had higher root quality compared to two leaves without influencing rooting success rate. Position of cutting on the stock plant: Did not influence either rooting success rate or root quality.


To maximize Rooting Success and Quality of Cuttings..
Take plant material from either apical or basal positions
Make sure cuttings have at least three fully expanded leaves
Do not cut leaves
Dip the cuttings in an IBA rooting hormone


Link to Lecture Slides: https://drive.google.com/file/d/1wZOO...

420 Magazine Source Content by Slide Title:
Many Variables
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Cannabis Plant Material and
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Propagation Environment
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Harvest Determination
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Rooting Assessment
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Research Summary
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