Single Molecule Spectroscopy - Chris Johnson

The LMB Biophysics Facility houses a wide range of state-of-the-art and in-house built instruments that enable the molecular characterisation of macromolecular size, shape, mass, stability, binding affinity, dynamics and interaction kinetics.

The facility is primarily intended for hands-on research by students, postdocs, and group leaders, who prepare samples and make measurements. Biophysics supports users in advice on experimental techniques and results, training and in collaborative research.

In this video Chris explains why single molecule measurement should be untaken, the practicalities involved and several applications using the current single-molecule set-ups in the LMB including FRET and fluctuation correlation spectroscopy (FCS). The last part of the video details a single molecule light scattering technique using the Refeyn iSCAT to measure masses.

The Biophysics Facility is headed by Chris Johnson, supported by Stephen McLaughlin.

Read more about the facility here: https://www2.mrc-lmb.cam.ac.uk/resear...

You can find more videos from the LMB 2021 Biophysics Techniques Lecture series here:
   • Introduction to Biophysical Technique...  

Other playlists you might be interested in:
Next Generation Biophysics Symposium 2020 -    • Next Generation Biophysics Symposium ...  
Electron Cryo-Microscopy Course 2017 -    • Electron Cryo-microscopy Course 2017  
RNA Seq Course -    • RNA-seq Course 2020  
LMB Crystallography Course 2013 -    • LMB Crystallography Course 2013  

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