Measuring Water Potential by Using Chardakov Method

Chardakov Method, Water Potential, Plant Physiology,
Universiti Malaysia Sabah

Introduction
The Chardakov's Method
Changes in Solution Density after Immersion

Methodology
Materials and Methods
Step 1: Preparing Sucrose Solution of Different Concentrations
Step 2: Putting Kenanga Leaf into 0.1 M Test Tube
Step 3: Immersing Leaf with 0.1 M Sucrose Solution
Step 4: Repeat Step 2 for Other Leaves
Step 5: Repeat Step 3 with Different Concentrations
Step 6: Taking Temperature of Immersed Set A Solutions
Step 7: Colour Set B Solutions with Methylene Blue
Step 8: Removing Leaves from Test Tubes
Step 9: Dropping Original into Immersed Solutions
Step 10: Observing Blue Drop Movements

Results
Observation of Solution Temperature and Drop Movement

Discussion
van's Hoff Equation

Conclusion

Credits