Скачать или смотреть Reproducibility Issues in Life Science Research: The Importance of Cell Line Authentication

Alarms about the shortcomings of many cell lines and their deleterious impact on experiment reproducibility and the scientific record have been ringing for over 50 years. Today, even though there is consensus among scientists that misidentified and contaminated cell lines are a significant problem, some labs are reluctant to authenticate their cell lines. But mistakes are being made and they are piling up. In 2015, Christopher Korch, Ph.D., from the University of Colorado, reported that nearly 5,800 articles in 1,182 journals may have confused HeLa for HEp-2, and another 1,336 articles in 271 journals may have mixed up HeLa with INT 407. This documentary discusses the concerns surrounding cell line identification and what can be done to alleviate the problem.