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A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example - a 2 minute Preview of the Experimental Protocol

Lise Schotte, Bart Rombaut, Bert Thys
Vrije Universiteit Brussel, Department of Pharmaceutical Biotechnology and Molecular Biology, Centre for Pharmaceutical Research;

In this article, a simple, quantitative, liquid phase affinity capture assay is presented. It is a reliable technique based on the interaction between magnetic beads and tagged proteins (e.g. nanobodies) on one hand and the affinity between the tagged protein and a second, labeled protein (e.g. poliovirus) on the other.

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