Скачать или смотреть [Conférence] M. DAHAN - In situ biochemistry with single molecule imaging

00:00:00 Introduction
00:00:35 Frommicroscopy to « nanoscopy »
00:02:34 Single Molecule Localisation Microscopy
00:03:50 Hallmarks of self-organizing supramolecular assemblies in cells
00:05:26 Single molecule imaging in live cells
00:06:18 Distribution of synaptic receptors in the neuronal membrane
00:07:22 Probing the interactions between glycine receptors and gephyrin scaffolds
00:08:10 Transient receptor-scaffold interactions
00:09:01 Gephyrin clusters act as trapping potentials
00:10:01 Stoechiometric analysis of gephyrin clusters
00:11:19 Number of gephyrin molecules
00:11:51 Correlation between the number of receptors and gephyrin molecules
00:14:03 3D astimagtic imaging using adaptive optics
00:14:48 3D astimagticimagingusing adaptive optics
00:16:07 3D super-resolution images of individual synapses
00:17:09 Quantitative nanoscopy of inhibitory synapses
00:17:29 Novel SPT Microscopy Methods
00:18:27 SPT Microscopy Methods
00:18:59 High-Density Single-Particle Tracking
00:19:36 From a few trajectories to “big” single molecule data
00:20:25 Bayesian inference Approach
00:22:17 Numerical simulations
00:22:54 Reconstitution of GlyR-gephyrin interactions using a TMD with an intracellular b-loop
00:23:30 Distribution of synapticreceptorsin the neuronal membrane
00:23:41 High-density single moleculeimaging in neurons
00:24:11 Energy traps coincide with gephyrin clusters
00:24:33 Reconstitution of GlyR-gephyrin interactions using a TMD
00:25:32 Receptor-Scaffold Interactions
00:26:16 Quantitative nanoscopy of inhibitory synapses
00:27:39 Conclusion and perspectives
00:31:09 Thanks
00:32:11 Questions


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