HPLC Troubleshooting Interview questions | HPLC Troubleshooting | Quality control | English Excel

This video contains most important and selective 20 HPLC Troubleshooting interview questions with answers........
Friends, this video is very helpful and knowledgeable for those who are regularly performing analysis on HPLC.
In this video i have selected most important questions on HPLC Troubleshooting which are frequently asked during interview.
Q1) What do you mean by Troubleshoot?
Q2) What are the six steps of troubleshooting process?
Q3) What causes RSD failure in HPLC?
Q4) What happens if your sample solvent is stronger than your mobile phase?
Q5) What to do when backpressure increases?
Q6) Tell me the 5 probable causes of variable retention times? (RT shift)
Q7) What are the cause of baseline drift or noise?
Q8) What to do when there are no peaks or small peaks?
Q9) What to do when there is no flow?
Q10) What are the probable causes of no Pressure/Pressure lower than usual?
Q11) What to do when Pressure higher than usual?
Q12) What are the causes of noisy baseline?
Q13) What are the causes of negative peak in your HPLC Chromatogram?
Q14) What causes the loss of resolution & how to solve?
Q15) What are the causes of spike baseline & how to solve it?
Q16) What are the causes of peak tailing?
Q17) What are the causes of Broad peaks & how to solve it?
Q18) What are the reasons of peak doubling?
Q19) What are the causes of peak splitting?
Q20) What are the types of baseline troubleshooting?

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