Secondary Metabolite production in plants

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This video lecture explains the topic secondary metabolite production in plants.
In this video following topics are covered:
Basics of Secondary Metabolite Production
Process involved in the secondary metabolite production like:
1. Developing Mother Culture
2. Selection of cell lines for high yield of secondary metabolites.
3. Large scale cultivation of plant cells
4. Medium composition and effect of nutrients
5. Elicitor induced production of secondary metabolites
6. Effect of environmental factors
7. Biotransformation using plant cell culture
8. Secondary metabolites and analysis.

Large Scale Cultivation of Plant Cells
To achieve industrial production of the desired metabolite, large-scale cultivation of the plant cell is required. Plant cells when cultured exhibit changes in volumes and thus variable shapes and sizes. Further, cultured cells have low growth rate and genetic instability. All these aspects have to be considered for the mass cultivation of cells. the following four different culture systems are widely used:

1. The Free-Cell Suspension Culture
2. Immobilized Cell culture
3. Two Phase System culture
4. Hairy root culture

Bioreactors for secondary metabolite production in plants:

Batch Bioreactors
Continuous Bioreactors
Multistage Bioreactors
Airlift Bioreactors
Stirred Tank Bioreactors

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