Скачать или смотреть Advancing Drug Discovery: Cell Painting on the Araceli Endeavor® Platform

Advancing Life Sciences with Araceli Endeavor & Cell Painting

Join Martin Theiss, PhD., Application Scientist at Araceli Biosciences, and Fernanda García-Fóssa, PhD., Postdoctoral Scientist Max Planck Institute, as they share cutting-edge insights on cell painting technology using the Araceli Endeavor® platform.

This presentation covers:
The power of high-content imaging for phenotypic screening
How Dr. Fossa performed a cell painting assay 30 times faster than in previous attempts using the Araceli Endeavor® platform and CellProfiler™ high-content analysis software
How cell painting drives drug discovery and mechanistic insights
Best practices for data acquisition and analysis in modern life science workflows

Whether you’re in biotech, pharma, or academic research, this talk will give you tools to accelerate discoveries, improve data quality, and explore complex biological systems in greater detail.

Learn More: https://www.aracelibio.com
LinkedIn Page:   / araceli-biosciences  

0:00 - Intro
0:45 - Compound Management And Screening Center (COMAS)
2:36 - Summary
2:50 - The Cell Painting Assay
3:55 - Validate Cell Painting on Endeavor®
4:52 - Automated Cell Plating
5:15 - Cell Treatment Workflow With Echo System
6:03 - Cell Painting: Stain and Acquire
7:29 - Negative Controls: Endeavor® vs. Prior Imaging System
8:35 - Image Paralell Processing
9:17 - Segmentation
9:40 - Set of Features
10:29 - Aggregate Data
10:55 - Feature Selection
12:23 - Z-Scores
13:10 - Metrics
13:40 - Metrics: Biosimilarity
13:56 - Compounds Tested
16:09 - Benchmark Endeavor® Profiling's Ability
17:01 - Validate Cell Painting on Endeavor®
17:11 - CellProfiler™ Versions & Feature Consistency
17:48 - 23% of Correlation Features Have Low Pearson Scores
18:23 - Validate Cell Painting on Endeavor
18:35 - Endeavor® vs Legacy Imaging: Clustering
19:18 - Remove RNA Channel Impacts Performance?
19:36 - ER+RNA Channel vs. ER+AGP
20:40 - More Features ≠ Better Clustering
21:24 - Endeavor® With Robust Set of 579 Features
22:11 - Endeavor®: High Frequency of AreaShape Features
23:08 - Simulated Binning Yeilds Clustering
24:13 - What We Learned
24:54 - Key Takeaways
25:53 - Acknowledgments
26:15 - Araceli Endeavor® Portfolio
27:07 - Araceli Software Solutions
27:53 - Araceli Endeavor® Ultra Use Case: Ultra High-Throughput Screening
28:33 - Araceli Endeavor® Pro Use Case: High-Throughput functional Screening
29:18 - Araceli Endeavor® Core Use Case: Fixed-Cell Assay and Method Optimization
30:00 - Outro

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