Скачать или смотреть DNA-binding proteins as potential drug targets in Pseudomonas aeruginosa, Madeleine Hardie Boys

Pseudomonas aeruginosa is a top priority bacterium, causing infections in the urinary tract, lungs, and wounds. It causes especially high death rates in people with cystic fibrosis and is highly resistant to multiple antibiotics. It has a variety of mechanisms that contribute to its high drug resistance and infectivity, including the ability to clump together and adhere to surfaces such as the airways or medical equipment. The aim of this research project was to delete two genes, hypothesised to be involved in these mechanisms, and analyse the effects of these deletions on the bacterium. If the deletions reduced infectivity, then these genes are possible new drug targets. A screening process identified potential isolates with both genes deleted, but confirmation tests revealed that they had reverted to a wild-type phenotype.