Скачать или смотреть How does flash chromatography work?

Flash chromatography is a widely established chemical separation technique based on the polarity differences of compounds. General the following will influence the efficiency and success of your separation and purification:

1- Methodology
2- Theory of separation
3- Choice of solvent
4- Loading the column
5- Solvent equilibration
6- Loading of the sample
7- Column capacity

To know more about the principal common steps listed above and some rules of thumb for performing flash chromatography read this Application Note:
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Have more questions about Flash Chromatography or about the sorbents you can use?
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Prior to performing a separation with a chromatographic column, it is highly recommended to perform screening tests using thin layer chromatography (TLC) to determine the best conditions for the separation.
To understand the relationship and how one transfers to the other, this video in our Q&A series is great:    • FAQ What is the relationship between reten...