🔬 How to get a 3D effect with your microscope | Amateur Microscopy

Oblique Illumination is an easy way to get a 3D effect using your microscope. It can be achieved by blocking the light on one side of the microscope lamp. The specimen is then lighted from the side. While the quality is not as high as from DIC (differential interference contrast), the big advantage is its low cost. For best results, it is important to very carefully align the oblique illumination filter.

Pictures comparing DIC and oblique illumination: In this German microscopy forum a user compared these two techniques with a side-by-side comparison of pictures (scroll down). The pictures labelled "DIK" are made using a DIC microscope and thoese labelled with "Schiefe Bel." are made using oblique illumination.
https://www.mikroskopie-forum.de/inde...

For a more theoretical and in-depth discussion of the technique:
https://www.microscopyu.com/technique...
https://www.olympus-lifescience.com/e...

Some historical background of oblique illumination:
http://www.microscopy-uk.org.uk/dww/a...

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