A hybrid model of Blastocystis AOX - Video abstract: 26820

Video abstract of original research paper "Modeling the alternative oxidase from the human pathogen Blastocystis using automated hybrid structural template assembly" published in the open access journal Research and Reports in Biochemistry by Daron M Standley and Mark van der Giezen.

Read the full research paper here: http://www.dovepress.com/modeling-the...

Abstract: Alternative oxidases (AOX) of human parasites represent attractive drug targets due to their absence in humans. However, the lack of a structure has prevented structure-based drug design. Moreover, a large helical insertion proves difficult for automated structural modeling efforts. We have used a novel hybrid structural modeling approach to generate a model that is globally consistent with a previous model but based on a phylogenetically closer template and systematic sampling of known fragments in the helical insertion. Our model, in agreement with site-directed mutagenesis studies, clearly assigns E200 as the iron-ligating residue as opposed to the previously suggested E201. Crystallization of AOX from another species has recently been reported suggesting that our blind prediction can be independently validated in the near future.