Скачать или смотреть Single-Cell Phenoscaping of Tumor Microenvironment Organoids

Speaker: Chris Tape, PhD, Associate Professor, UCL Cancer Institute Cancer cells are regulated by oncogenic mutations and microenvironmental signals, yet these processes are often studied separately. To functionally map how cell-intrinsic and cell-extrinsic cues co-regulate cell-fate, Tape and his team performed a systematic scRNA-seq and mass cytometry analysis of 1,107 colonic organoid cultures. They discovered a stepwise epithelial differentiation phenoscape dictated by combinations of oncogenes and stromal ligands, spanning from fibroblast-induced revival colonic stem cells (revCSC) to oncogene-driven hyper-proliferative CSC (proCSC).

Recorded on May 3, 2024

#organoids #masscytometry