Скачать или смотреть The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

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This article aims to provide researchers with an alternative method for preparing primary hematopoietic cell cultures from murine bone marrow for electroporation. The traditional approach to isolating and cultivating these cells can be challenging and may result in impure populations. Therefore, this article offers a step-by-step procedure that ensures the successful isolation and cultivation of hematopoietic cells.

By following this method, researchers can obtain a pure population of hematopoietic cells that can be utilized for various experimental purposes. For instance, these cells can be used in gene transfer studies, where researchers can introduce specific genes into the cells to study their effects on hematopoiesis. Additionally, these primary cell cultures can be employed in drug screening assays, allowing researchers to test the efficacy and toxicity of potential therapeutic agents on hematopoietic cells.

The protocol outlined in this article includes detailed instructions on bone marrow extraction, which is a critical step in obtaining hematopoietic cells. Researchers will learn how to properly isolate the bone marrow from murine bones, ensuring minimal contamination and damage to the cells. The article also provides guidance on the subsequent cell isolation process, where researchers will learn how to separate hematopoietic cells from other cell types present in the bone marrow.

Furthermore, the article offers comprehensive information on the optimal culture conditions required for the successful growth and maintenance of primary hematopoietic cell cultures. Researchers will gain insights into the appropriate media composition, growth factors, and cytokines necessary for promoting the proliferation and differentiation of hematopoietic cells. This knowledge will enable researchers to create an ideal environment for the cells to thrive and exhibit their natural functions.