Скачать или смотреть Formalin (Formaldehyde Fixative) ; Definition, Properties, Mechanism, Application, Histology

Formalin is a widely used fixative in histology and pathology, primarily composed of 37-40% formaldehyde gas dissolved in water. It is often diluted to a 10% formalin solution (equivalent to 4% formaldehyde) for tissue fixation. Here's an overview:

Key Features:
Mechanism:

Formalin cross-links proteins by reacting with amino groups, preserving tissue structure and preventing autolysis and putrefaction.

It stabilizes cellular components, making tissues suitable for further processing and staining.

Applications:

Commonly used in routine histology for preserving tissues.

Suitable for immunohistochemistry and other staining techniques.

Advantages:

Penetrates tissues quickly and fixes them effectively.

Preserves tissue morphology and antigenicity for diagnostic purposes.

Limitations:

Prolonged fixation can lead to over-hardening of tissues.

Formaldehyde vapors are toxic and require proper handling and ventilation.

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