This video is the complete 2-day recording of the Molecular Biology Techniques Masterclass organized by Zynome Scientific. It provides a thorough, practical, and easy-to-understand journey through the core techniques used in modern molecular biology laboratories. The workshop begins with the fundamentals of molecular biology and gradually builds toward advanced gene expression techniques, making it suitable for students, researchers, and professionals at all levels. Throughout the sessions, you will learn how DNA is extracted from cells and tissues, how purity and concentration are measured using spectrophotometric methods, and how agarose gel electrophoresis is performed and interpreted. The workshop also covers essential cloning-related techniques such as restriction digestion and DNA ligation, with clear explanations of mechanisms, workflow, and troubleshooting.
As the masterclass progresses, the focus shifts toward gene manipulation and expression analysis. You will gain a solid understanding of PCR—how it works, what components are required, how optimization is performed, and how different factors affect amplification. The recording also demonstrates reverse transcription and cDNA synthesis, the complete workflow of bacterial transformation, and the principles behind quantitative real-time PCR (qPCR), including Ct values, amplification curves, and fold change calculations. A dedicated part of the masterclass focuses on primer designing, covering rules, common mistakes, practical examples, and the use of online tools for accurate design. Participants are also introduced to useful in-silico resources such as BLAST and the UCSC Genome Browser, helping bridge the gap between wet-lab experiments and bioinformatics.
By watching this masterclass, you will gain not only theoretical knowledge but also practical confidence, troubleshooting strategies, and a clear understanding of how different molecular techniques connect to each other within a real research workflow. Whether you are preparing for laboratory work, academic exams, research projects, or professional development, this detailed workshop offers a complete foundation in molecular biology techniques. Organized by Zynome Scientific with the mission of empowering researchers through high-quality education, this masterclass brings together essential concepts, live demonstrations, and real-world insights to help you strengthen your skills and advance in the field of molecular biology.
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