Скачать или смотреть India ink positive ||Cryptococcal Meningitis

||India Ink Preparation||
This test is ordered in suspected case of cryptococcal meningitis.
Principle
The capsule is non ionic, so that the  India ink  used will not bind to it. This is reason, capsule appears as a clear halo around the yeast cells.

Requirements
India ink

slide and cover slip

C.S.F. specimen

Droppers or inoculating loop

waste discarding container or Bunsen burner

Centrifuge

test tubes

Procedure
1. Centrifuge the C.S.F. for 5 to  10 minutes,

2. Remove the supernatant fluid and mix the sediment.

3. Transfer  equal amount of  sediment and India ink i.e. a drop of the sediment to a slide and add a drop of India ink .

4. Mix and cover with a  cover slip.

5. Now, examine the preparation under microscope using the 40X objective.

Observation
Look for oval or round cells, some showing budding, irregular in size, measuring 2-10 µm in diameter and surrounded by  a large unstained capsule as shown in above figure. Very rarely capsules are absent.
For more details, go with website @
http://universe84a.com/india-ink-prep...

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