In Vivo Calcium Imaging of Learning Induced Synaptic Plasticity | Protocol Preview

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In Vivo Optical Calcium Imaging of Learning-Induced Synaptic Plasticity in Drosophila melanogaster - a 2 minute Preview of the Experimental Protocol

Clare E. Hancock, Florian Bilz, André Fiala
University of Göttingen, Department of Molecular Neurobiology of Behavior;

Here we present a protocol with which pre- and/or postsynaptic calcium can be visualized in the context of Drosophila learning and memory. In vivo calcium imaging using synaptically localized calcium sensors is combined with a classical olfactory conditioning paradigm such that the synaptic plasticity underlying this type of associative learning may be determined.

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