Mitochondrial Calcium Influx Analysis | Protocol Preview

Watch the Full Video at https://www.jove.com/v/57225/analyses....

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells - a 2 minute Preview of the Experimental Protocol

Joshua T. Maxwell, Chin-Hsien Tsai, Tahmina A. Mohiuddin, Jennifer Q. Kwong
Emory University School of Medicine, Department of Pediatrics; Emory University, Emory College of Arts and Sciences;

Here, we present two protocols for the measurement of mitochondrial Ca2+ influx in isolated mitochondria and cultured cells. For isolated mitochondria, we detail a plate reader-based Ca2+ import assay using the Ca2+ sensitive dye calcium green-5N. For cultured cells, we describe a confocal microscopy method using the Ca2+ dye Rhod-2/AM.

Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.

Subscribe to our channel:    / jovejournalofvisualizedexperiments