Identification of Frequent Autoreactive B-cell Response in Pancreatic Ductal Adenocarcinoma Using...

Presented By: Min Yao, Ph.D. & Patrick Murphy, Ph.D.
Speaker Biography:
Min Yao, Ph.D., is a senior research scientist at the Sanders Tri-institutional Therapeutics Discovery Institute, a collaborative effort between Memorial Sloan Kettering Cancer Center, The Rockefeller University, and Weill Cornell Medicine in New York. Dr. Yao specializes in antibody development and discovery, leveraging advanced techniques such as antigen-specific B cell sorting and single-cell sequencing. Previously, he conducted postdoctoral research at Cold Spring Harbor Laboratory, focusing on studying B cell response in pancreatic cancer under the mentorship of Dr. Douglas Fearon. Dr. Yao earned his doctoral degree from the University of Kansas Medical Center, where he investigated breast cancer progression under the guidance of Dr. Nikki Cheng.

Patrick is a Senior Science and Technology Advisor for 10x Genomics. He completed his PhD in Immunology at the University of Rochester, where he studied the impact of apoptotic cell clearance in macrophage inflammatory responses. Following that, he worked at the University of Pittsburgh as a research assistant professor studying tumor infiltrating lymphocytes (TIL) as a treatment for metastatic uveal melanoma. During his time at Pitt, Patrick made extensive use of single-cell technologies. Subsequently he joined 10x Genomics, where he supports these technologies in his current position.

Webinar: Identification of Frequent Autoreactive B-cell Response in Pancreatic Ductal Adenocarcinoma Using Cell Sorting and Single-Cell Sequencing
Webinar Abstract:
B cell responses within pancreatic ductal adenocarcinoma (PDAC) tumors are associated with improved patient outcomes, yet the specific triggering antigens remain largely unknown. In this webinar, Dr. Min Yao will present a methodology that combines cell sorting, single-cell RNA sequencing (scRNA-Seq), and immunoglobulin (Ig) sequencing of tumor-infiltrating immune cells to uncover these antigens. Dr. Yao's study shows evidence of various immune responses, including activated T and B cells, germinal center reactions, and plasma cells producing antibodies targeting intracellular self-antigens in pancreatic cancer tissue. These findings indicate a potential link between chronic inflammation in pancreatic cancer and an enhanced adaptive immune response, which could have implications for treatment development.

Learning Objectives
Understand the immune micro-enviorment in pancreatic ductal adenocarcinoma (PDAC) using the SH800 Cell sorter and 10x Genomics single-cell analysis tools
Learn how to identify specific antigens associated with B cell responses in clinical samples
Gain insights on autoreactive B cell responses in PDAC and its potential clinical impact

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