52.Restriction mapping and Pulsed Field Gel Electrophoresis (PFGE) | شرح بالعربي Genomics

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00:00 introduction
01:11 Why do we need to make physical mapping?
04:57 Genetic map vs. physical map
10:30 What is restriction mapping?
17:14 Limitations of restriction mapping
20:17 How to solve this limitation
23:56 Disadvantages of the conventional gel electrophoresis
27:02 Pulsed Field Gel Electrophoresis (PFGE)
28:53 Field Inversion Gel Electrophoresis (FIGE) Vs. Counter Clamped Homologous Gel Electrophoresis (CHEF)
33:30 DNA isolation using immobilized cells in agarose blocks