Скачать или смотреть Dr Beren Atac: 3D Micro Hair Follicle Culture In a Dynamic Chip Bioreactor

Presented at the 8th World Congress for Hair Research (2014) in Jeju Island, South Korea.

Background:
Recently, we developed an in vitro hair follicle equivalent termed micro-follicle (MF). This 3D hair follicle model is produced under ultra-low attachment conditions, mimicking mesenchymal condensation during embryonic development and subsequent keratinocytes differentiation. At early stages, the model is established by the self-aggregation of dermal papilla (DP) cells forming neopapillae which are envisioned for hair transplantation procedures due to their inductive capacity. For micorfollicle formation, the model is composed of cells from additional germ layers including keratinocytes, melanocytes and endothelial cells.

Objectives:
Nutrient supply in vitro is limited with the size of the organoid and ultimately effects differentiation efficiency of tissues under static conditions. The multi-organ-chip (MOC) platform is a micro-scale bioreactor providing pulsatile dynamic perfusion for micro-scale organoids.

Recently, we adapted the MF culture to the dynamic chip platform for the further development of organoids and following substance testing in a new 3D matrix environment.

Material & Methods:
Poly-carbonate (PC) based micro thermo-formed 3D cell culture material is used to adapt MF culture to the MOC platform. This PC based material is formed as micro-cavitites with latent tracks providing perfusion from each dimension. MFs are dynamically cultured for 14 days in the MOC and immunohistology is performed as end point analysis to characterise different morphological changes.

Results:
MFs respond to the surface and geometry of the material and the thermo-formed PC enables dynamic culture of MF for more than 14 days. The formed tissues showed polarisation and hair follicle-related expression patterns.

Discussions & Conclusion:
Thermo-formed PC with its special micro cavity structure is a suitable material for MF cultures providing geometry for 3D tissue, a basis for polarisation as well as allowing nutrient supplementation through perfusion into the MOC. This dynamic culture model provides a suitable system for hair related substance testing.