Скачать или смотреть Digital PCR vs qPCR: A Game Changer in DNA Analysis.

Now let's compare digital PCR with its predecessor, qPCR. Both techniques amplify DNA, but their methods differ significantly. QPCR monitors the amplification process in real time, measuring the amount of DNA based on fluorescence intensity. It's like estimating the number of fireflies in a field by how bright the collective light is. Digital PCR, on the other hand, counts individual DNA molecules after the amplification process. This fundamental difference in approach gives digital PCR a significant edge in sensitivity and accuracy, particularly when dealing with rare DNA sequences or small variations in gene expression. Furthermore, qPCR relies on standard curves generated from known DNA concentrations. Digital PCR requires no standard curves, offering absolute quantification and reducing the risk of errors. In short, while qPCR remains valuable, digital PCR offers superior precision, sensitivity, and absolute quantification.

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