Скачать или смотреть Time-lapse microscopy of mouse cortical axons (5 days)

The mouse cortical neurons are sparsely labeled with green fluorescent protein (GFP). Only ~5% of the total neurons within the field of view are visible. For this movie, image resolution was reduced about 5x to facilitate easy display; the original size of each frame is about 30 megapixels (with a single pixel width of 167nm). To achieve such high resolution over a large area, each movie frame is a mosaic image of 5x5 individual tiles. The entire field of view (~1mm^2) was imaged every 10 minutes continuously over the five day period. One second in the movie equals one hour of realtime. The red scale bar in the upper right corner is 50um.

This data was collected by Neville Sanjana in Sebastian Seung's lab as part of Neville's PhD thesis in Neuroscience. Thesis title: Quantitative analysis of axon elongation: A novel application of stochastic modeling techniques to long-term, high-resolution time-lapse microscopy of cortical axons.

A downloadable copy of the thesis is available from MIT DSpace: https://dspace.mit.edu/handle/1721.1/...

This movie also won an award at the Olympus BioScapes International Digital Imaging Competition in 2009:
http://www.olympusbioscapes.com/galle...
http://www.olympusbioscapes.com/galle...