Multiplex PCR solutions from DNA Software

PanelPlex™ designs up to 100-plex in hours not months with more than 95% success. Automates designs for PCR primers with highly uniform amplification all of targets. Can design in the presence of existing panels.
VisaulOMP is a multiplex PCR primer design software that can design multiple primers and probes at the same time using a dynamic design algorithm that queries each oligo design against every other oligo in the system to check for intended and unintended cross- and mishybridization. The size of the plex design depends on many factors such as target number, length, complexity and secondary structure, all of which further depends on the defined experimental conditions that are used. A typical use case would involve a 20-plex of targets that are about 500 nucleotides long at standard endpoint PCR conditions. Multiplexes that are larger than this would require the use of OMP-Developers Edition, which is a server-based command line design and simulation application.

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