Скачать или смотреть Applied Genomics - QC in Genomics: Dr. Steve Jackson, Dr. Emily Zeringer & Dr. Harita Veereshlingam

Presented By: Dr. Steve Jackson, Dr. Emily Zeringer & Dr. Harita Veereshlingam

Speaker Biography:

Steve Jackson, PhD
Senior Manager, Product Applications - Thermo Fisher Scientific

Harita Veereshlingam, PhD
Senior Product Application Scientist, Genetic Sciences Division - Thermo Fisher Scientific

Emily Zeringer, PhD
R&D Staff Scientist, Sample Preparation BioSciences, ThermoFisher Scientific, USA

Webinar:

Strategies to normalize miRNA expression in serum and plasma

Authenticating human cells: Knowing exactly what is in your test tube

Webinar Abstract:

MicroRNA(miRNA) are short non-coding single stranded RNA molecules that regulate gene expression at the post transcriptional level.  They are known to play a critical role in multiple biological processes including proliferation, differentiation, apoptosis and hematopoiesis. Dysregulation of miRNA expression therefore is associated with a number of pathological conditions such as inflammation, cardiovascular diseases, neurological disorders and several types of cancers. miRNAs are also found to be extremely stable in body fluids such as serum, plasma, urine, breast milk and saliva...

The study of human development and diseases relies on analysis of samples obtained and manipulated ex vivo. It is critical in such studies to know the provenance and confirm that the identity of these materials is correct. Analysis of highly variable short tandem repeats (STRs), provides a simple, inexpensive and highly specific genetic “fingerprint” of a human sample.  In this study, we describe a complete workflow for cell line authentication, chimeric receptor T-cell (CAR-T) and induced pluripotent stem cell (iPSC) sample matching.  Analysis of serial dilutions of purified genomic DNA from isolated human cell lines identified the correct alleles from as little as 0.1ng of purified DNA.  Samples immobilized onto Copan NUCLEIC Cards were correctly identified when a suspension of 5-10 x 105 cells were dried onto the cards.  Contaminating HeLa alleles could be detected in mixture when they were present in as little as 1% of genomic DNA from this population. ..