Basics and principle of Fluorescence & Phosphorescence measurement | Learn under 5 min | AI 06

Analytical Instrumentation - Fluorescence & Phosphorescence measurement
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You may have come across terms like fluorescence and phosphorescence. You may have a lot of questions in your head regarding this topic like: What does fluorescent mean?, How does fluorescence works?, How does fluorescence spectroscopy work?, How does fluorescent occur? You will find answers to all these questions in this video.

In the following video, viewers will learn about the basic principle of fluorescence and phosphorescence. The generation of fluorescent and phosphorescent emissions is discussed in detailed using an energy level diagram of a molecule. In the energy level diagram we can see different levels of molecular energies. The electrons when in pair are either in singlet state or triplet state. The basic definitions of singlet state and triplet state is covered and pairing of electrons and their spins is explained in the animation. Once we understand the energy levels corresponding to singlet and triplet state, it becomes quite clear to understand fluorescence and phosphorescence. The fluorescence and phosphorescence emitted by a sample under test is used to perform qualitative and quantitative analysis on the analyte. It is performed using spectrofluorimeter. The basic principle of measurement of fluorescence is also explained in the video.

At the end of the video, viewer will also have a clear idea between the differences in fluorescence and phosphorescence and will be able to compare the two topics.
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